p28 azurin peptide sequence sequence - peptides-as-multifunctional-players-in-cancer-therapy p28 Unveiling the P28 Azurin Peptide Sequence: A Promising Anticancer Agent

peptides-are-legal The p28 azurin peptide sequence has emerged as a significant area of research within the field of oncology, offering a novel approach to cancer treatment. This 28 amino acid peptide, derived from the bacterial protein azurin, specifically from amino acids 50 to 77 of the full azurin molecule, exhibits potent anticancer properties. The precise p28 sequence is LSTAADMQGVVTDGMASGLDKDYLKPDD, with a molecular weight of approximately 2914Azurin p28 peptide.18 Da. This peptide has garnered attention for its ability to penetrate tumor cells and its mechanism of action involving the critical tumor suppressor protein, p53.

Azurin, a redox protein secreted by various bacteria, including *Pseudomonas aeruginosa*, is a 128 amino acid Cupredoxin. The p28 peptide represents a fragment of this larger protein, specifically the sequence spanning from Leucine 50 to Aspartic acid 77 (Leu50–Asp77). Research indicates that this peptide is preferentially taken up by human breast cancer cell lines, highlighting its potential as a targeted therapeutic agent.p28is derived fromAzurin, a 128 amino acid Cupredoxin, which is secreted by Pseudomonas aeruginosa. Interestingly,p28is an anionic cell-penetratingpeptide...

A key aspect of the p28 azurin peptide's efficacy lies in its interaction with the p53 protein. p53 is a vital protein that plays a crucial role in preventing cancer by regulating cell division and initiating programmed cell death (apoptosis) in damaged cells.p28 Bacterial Peptide, as an Anticancer Agent - PMC However, in many cancers, p53 function is compromised, often due to its degradation by cellular mechanisms. The p28 peptide acts as a first-in-class peptide inhibitor that disrupts the interaction between p53 and its negative regulators, such as HDM2 (also known as MDM2) and COP1. By binding to p53, the p28 peptide forms a p28:p53 complex, which prevents the proteasomal degradation of p53. This stabilization of p53 allows it to perform its tumor-suppressive functions more effectively, leading to reduced tumor growth and potentially inducing apoptosis.

Studies have investigated the solution structure of the anticancer p28 peptide, revealing insights into its conformational properties that contribute to its therapeutic potential. The p28 peptide is described as a water-soluble, amphipathic peptide, further supporting its ability to interact with cellular components.Azurin p28 peptide | 抗肿瘤肽 The precise order of amino acids in the p28 sequence is critical for its binding affinity and inhibitory activity.

The development of the p28 peptide has progressed through various stages, including preclinical studies and early-phase clinical trialsConstruction, Expression, and Purification of p28 as a Cell- .... A first-in-human trial of the azurin-derived P28 peptide has been conducted, assessing its safety and potential efficacy. The purity of the Azurin p28 peptide is typically ≥95%, ensuring its suitability for therapeutic applications.

Further research continues to explore the nuances of the p28 azurin peptide sequence and its interactions. For instance, the role of specific amino acid residues within the p28 sequence, such as those in the range of 1–4, 7–11, and 22–23, have been implicated in masking the hydrophobic pocket of HDM2, thereby disrupting the p53–HDM2 interactions.Azurin p28 peptide | p53: Tocris Bioscience Analysis of p28 sequences from different bacterial species is also underway to understand variations and potentially identify optimized peptide P28 variants.

In summary, the p28 azurin peptide sequence represents a significant advancement in the search for novel cancer therapies. Its ability to stabilize p53 by inhibiting its degradation pathways, coupled with its tumor-penetrating properties, positions it as a promising anticancer agent作者：T Yamada·2013·被引用次数：116—Background: A 28 amino-acid (aa) cell-penetratingpeptide(p28) derived fromazurin... MolecularSequenceData;PeptideFragments .... Ongoing research into its sequence, structure, and mechanism of action continues to unlock its full therapeutic potential. The Azurin p28 is a testament to the power of leveraging natural peptides for biomedical innovation.